CCL3 Kit ELISA
Aperçu rapide pour CCL3 Kit ELISA (ABIN5026947)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 23.44 pg/mL
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Fonction
- This immunoassay kit allows for the in vitro quantitative determination of Human MIP-1Alpha /CCL3 concentrations in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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Sensibilité
- < 14 pg/mL
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Ingrédients
- plate, standard, diluent, antibodies
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Matériel non inclus
- pipettes, tubes, reader, incubator
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Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA Kit
CCL3 Reactivité: Souris Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Humain Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Chien Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Souris AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Rat AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Souris Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Lapin Colorimetric 7.8-500 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Humain AA 27-92 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 3 (CCL3) ELISA KitCCL3 Reactivité: Souris AA 33-262 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Tissue Homogenate
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 - 4 h
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Plaque
- Pre-coated
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Protocole
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human MIP-1Alpha /CCL3 antibody was pre-coated into 96-well plates. Biotin conjugated anti-human MIP-1Alpha /CCL3 detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MIP-1Alpha /CCL3 amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human MIP-1Alpha /CCL3 can be calculated using the standard curve.
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Restrictions
- For Research Use only
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Précaution d'utilisation
- protect your eyes
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Stock
- 4 °C
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- CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))
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Autre désignation
- MIP-1alpha/CCL3 (Macrophage Inflammatory Protein-1alpha)
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Autophagy
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